Products recommended by Tongrenxin TB-IGRA
1、 Overview
Tuberculosis- γ The principle of interferon release test (TB-IGRA) is as follows:
The specific stimulating antigens "esat6 and cfp10" are unique fragments of pathogenic Mycobacterium tuberculosis but not of BCG and other mycobacteria.
Mycobacterium tuberculosis infected persons have specific T lymphocytes for each antigen, which can recognize and present these antigens, stimulate the proliferation of T lymphocytes, and release cytokines, such as IFN- γ。 Specific IFN released in vitro can be quantitatively detected by ELISA- γ， So as to judge whether it is infected with Mycobacterium tuberculosis.
2、TB-IGRAprocess
A、Schematic diagram of stimulation process:
B、Schematic diagram of detection process:
3、 Recommended usage（ELISA）
A、Amount of stimulator: 1ug/tube
B、Recommendations for use of ELISA reagents:
C6H4：1:1000Coated with antibody overnight. Washing and sealing. Sampling100ul，Joining at the same time20ul 1:2000Diluted8A11-F(ab)2-HRP，Washing, color development and termination.
C、Quantitative range of standard
Take the mean absorbance of the calibration sample as the independent variable（X），Its correspondingIFN-γContent of（12.5 pg/ml~400 pg/ml）Is a dependent variable（Y），The linear regression equation is obtained by power fittingy=167.64x0.9554，The data and schematic diagram are as follows：
校准品值 | 2.294 | 1.277 | 0.621 | 0.275 | 0.136 | 0.065 |
校准品IFN-γ含量 （单位：pg/ml） | 400 | 200 | 100 | 50 | 25 | 12.5 |
4、 Result judgment recommendation
Test culture tube (T) content value=T, background control culture tube (N) content value=N, positive control culture tube (P) content value=P (unit: pg/ml)
N | P-N | T-N | 结果判定 | 结果解释 |
≤400 | 任何值 | ≥14 并且≥(N/4) | 阳性 | 感染结核分枝杆菌 （结核活动期感染、潜伏感染或隐性感染） |
≥20 | <14 | 阴性 | 未感染结核分枝杆菌 | |
≥20 | ≥14但<(N/4) | 阴性 | ||
<20 | <14 | 不确定 | 不能确定是否感染结核分枝杆菌 | |
<20 | ≥14但<(N/4) | 不确定 | ||
>400 | 任何值 | 任何值 | 不确定 |
5、 Performance evaluation
*Refer to Australian QFT reagent
6、 Raw material information
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